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Dansk Selskab til Studiet af Parodontologi blev stiftet 23. januar 1953, som den første parodontologiske forening i Danmark.


Formålet med foreningen er at styrke kendskabet til sygdomsudvikling og behandling af marginal parodontitis samt peri-implantitis.
Foreningen har tandlæger som medlemmer og tandplejere som associerede medlemmer, hovedsageligt fra Danmark. Foreningen har i dag små 200 aktive medlemmer.

Foreningen afholder årligt 4 foredrag samt en generalforsamling. Inden hvert foredrag er der en sammenkomst med et lille traktement. Ved generalforsamling, som afholdes i marts/april, er der en middag.

Metaproteomics of saliva identifies human protein markers specific for individuals with periodontitis and dental caries compared to orally healthy controls.

Metaproteomics of saliva identifies human protein markers specific for individuals with periodontitis and dental caries compared to orally healthy controls.

Belstrøm D1Jersie-Christensen RR2Lyon D3Damgaard C4Jensen LJ3Holmstrup P1Olsen JV2.

 Abstract

BACKGROUND:

The composition of the salivary microbiota has been reported to differentiate between patients with periodontitis, dental caries and orally healthy individuals. To identify characteristics of diseased and healthy saliva we thus wanted to compare saliva metaproteomes from patients with periodontitis and dental caries to healthy individuals.

METHODS:

Stimulated saliva samples were collected from 10 patients with periodontitis, 10 patients with dental caries and 10 orally healthy individuals. The proteins in the saliva samples were subjected to denaturing buffer and digested enzymatically with LysC and trypsin. The resulting peptide mixtures were cleaned up by solid-phase extraction and separated online with 2 h gradients by nano-scale C18 reversed-phase chromatography connected to a mass spectrometer through an electrospray source. The eluting peptides were analyzed on a tandem mass spectrometer operated in data-dependent acquisition mode.

RESULTS:

We identified a total of 35,664 unique peptides from 4,161 different proteins, of which 1,946 and 2,090 were of bacterial and human origin, respectively. The human protein profiles displayed significant overexpression of the complement system and inflammatory markers in periodontitis and dental caries compared to healthy controls. Bacterial proteome profiles and functional annotation were very similar in health and disease.

CONCLUSIONS:

Overexpression of proteins related to the complement system and inflammation seems to correlate with oral disease status. Similar bacterial proteomes in healthy and diseased individuals suggests that the salivary microbiota predominantly thrives in a planktonic state expressing no disease-associated characteristics of metabolic activity.

PeerJ. 2016 Sep 14;4:e2433. doi: 10.7717/peerj.2433. eCollection 2016.

Link til artiklen: https://www.ncbi.nlm.nih.gov/pubmed/27672500

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In vitro complement activation, adherence to red blood cells and induction of mononuclear cell cytokine production by four strains of Aggregatibacter actinomycetemcomitans with different fimbriation and expression of leukotoxin.

In vitro complement activation, adherence to red blood cells and induction of mononuclear cell cytokine production by four strains of Aggregatibacter actinomycetemcomitans with different fimbriation and expression of leukotoxin.

Damgaard C1,2Reinholdt J3Palarasah Y4Enevold C5,6Nielsen C7Brimnes MK6Holmstrup P5Nielsen CH5,6.

Abstract

BACKGROUND AND OBJECTIVE:

The periodontal pathogen Aggregatibacter actinomycetemcomitans has been proposed as pro-atherogenic, and complement-mediated adherence to red blood cells (RBCs) may facilitate its systemic spread. We investigated the ability of four strains of A. actinomycetemcomitans with differential expression of leukotoxin A (LtxA) and fimbriae to activate complement, adhere to RBCs and elicit cytokine responses by mononuclear cells (MNCs).

MATERIAL AND METHODS:

Aggregatibacter actinomycetemcomitans serotype b strains HK 921, HK 1651, HK 2092 and HK 2108 were fluorescence-labeled, incubated with human whole blood cells in the presence of autologous serum, and assessed for RBC adherence by flow cytometry and for capacity to induce cytokine production by cytometric bead array analysis. The levels of IgG to A. actinomycetemcomitans serotype b were quantified by ELISA, as was consumption of complement.

RESULTS:

The JP2 clone variants HK 1651 and, to a lesser extent, HK 2092, consumed complement efficiently, while HK 2108 (= strain Y4) consumed complement poorly. Nonetheless, the four tested strains adhered equally well to RBCs in the presence of autologous serum, without causing RBC lysis. The JP2 clone variant HK 2092, selectively lacking LtxA production, induced higher production of tumor necrosis factor (TNF)-α, interleukin (IL)-1β, IL-6 and IL-10 by MNCs than did the other three strains, while the four strains induced similar production of IL-12p70. RBCs facilitated the HK 2092-induced production of TNF-α and IL-1β, and IL-6 was enhanced by RBCs, and this facilitation could be counteracted by blockade of complement receptor 3 (CD11b/CD18).

CONCLUSION:

Our data suggest that the JP2 clone of A. actinomycetemcomitans, most closely resembled by the variant HK 1651, activates complement well, while strain Y4, represented by HK 2108, activates complement poorly. However, all strains of A. actinomycetemcomitans adhere to RBCs and, when capable of producing LtxA, prevent production of inflammatory cytokines by MNCs. This “immunologically silent” immune adherence may facilitate systemic spread and atherogenesis.

© 2016 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.

J Periodontal Res. 2016 Sep 24. doi: 10.1111/jre.12414. [Epub ahead of print]

Link til artiklen: https://www.ncbi.nlm.nih.gov/pubmed/27663487

 

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